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reporter plasmids for supertopflash  (Addgene inc)


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    Structured Review

    Addgene inc reporter plasmids for supertopflash
    (A): HEK293T cells were transiently transfected with firefly reporter plasmids for <t>SuperTopFlash</t> together with a fixed amount of each GFP tagged DVL1 construct or with a 1:1 stoichiometric ratio of two DVL1 constructs with the same total amount of DVL1 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (B): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each FLAG tagged DVL2 construct or with a 1:1 stoichiometric ratio of two DVL2 constructs with the same total amount of DVL2 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (C): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each HA tagged DVL3 construct or with a 1:1 stoichiometric ratio of two DVL3 constructs with the same total amount of DVL3 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. Individual p values were calculated with student t-tests and denote the difference between DVL-WT and the other conditions without WNT stimulation, difference between each condition with or without stimulation was also calculated. An asterisk indicates a significant difference: *: p < 0.05; **: p < 0.005; ***: p < 0.0005; ****: p < 0.0001.
    Reporter Plasmids For Supertopflash, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 552 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reporter plasmids for supertopflash/product/Addgene inc
    Average 96 stars, based on 552 article reviews
    reporter plasmids for supertopflash - by Bioz Stars, 2026-06
    96/100 stars

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    1) Product Images from "Pathogenic DVL frameshifting variants in Robinow syndrome disrupt WNT signaling and cellular dynamics"

    Article Title: Pathogenic DVL frameshifting variants in Robinow syndrome disrupt WNT signaling and cellular dynamics

    Journal: bioRxiv

    doi: 10.1101/2025.08.02.668297

    (A): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each GFP tagged DVL1 construct or with a 1:1 stoichiometric ratio of two DVL1 constructs with the same total amount of DVL1 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (B): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each FLAG tagged DVL2 construct or with a 1:1 stoichiometric ratio of two DVL2 constructs with the same total amount of DVL2 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (C): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each HA tagged DVL3 construct or with a 1:1 stoichiometric ratio of two DVL3 constructs with the same total amount of DVL3 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. Individual p values were calculated with student t-tests and denote the difference between DVL-WT and the other conditions without WNT stimulation, difference between each condition with or without stimulation was also calculated. An asterisk indicates a significant difference: *: p < 0.05; **: p < 0.005; ***: p < 0.0005; ****: p < 0.0001.
    Figure Legend Snippet: (A): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each GFP tagged DVL1 construct or with a 1:1 stoichiometric ratio of two DVL1 constructs with the same total amount of DVL1 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (B): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each FLAG tagged DVL2 construct or with a 1:1 stoichiometric ratio of two DVL2 constructs with the same total amount of DVL2 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (C): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each HA tagged DVL3 construct or with a 1:1 stoichiometric ratio of two DVL3 constructs with the same total amount of DVL3 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. Individual p values were calculated with student t-tests and denote the difference between DVL-WT and the other conditions without WNT stimulation, difference between each condition with or without stimulation was also calculated. An asterisk indicates a significant difference: *: p < 0.05; **: p < 0.005; ***: p < 0.0005; ****: p < 0.0001.

    Techniques Used: Transfection, Construct



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    reporter plasmids for supertopflash  (Addgene inc)
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    Addgene inc reporter plasmids for supertopflash
    (A): HEK293T cells were transiently transfected with firefly reporter plasmids for <t>SuperTopFlash</t> together with a fixed amount of each GFP tagged DVL1 construct or with a 1:1 stoichiometric ratio of two DVL1 constructs with the same total amount of DVL1 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (B): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each FLAG tagged DVL2 construct or with a 1:1 stoichiometric ratio of two DVL2 constructs with the same total amount of DVL2 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (C): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each HA tagged DVL3 construct or with a 1:1 stoichiometric ratio of two DVL3 constructs with the same total amount of DVL3 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. Individual p values were calculated with student t-tests and denote the difference between DVL-WT and the other conditions without WNT stimulation, difference between each condition with or without stimulation was also calculated. An asterisk indicates a significant difference: *: p < 0.05; **: p < 0.005; ***: p < 0.0005; ****: p < 0.0001.
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    (A): HEK293T cells were transiently transfected with firefly reporter plasmids for <t>SuperTopFlash</t> together with a fixed amount of each GFP tagged DVL1 construct or with a 1:1 stoichiometric ratio of two DVL1 constructs with the same total amount of DVL1 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (B): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each FLAG tagged DVL2 construct or with a 1:1 stoichiometric ratio of two DVL2 constructs with the same total amount of DVL2 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (C): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each HA tagged DVL3 construct or with a 1:1 stoichiometric ratio of two DVL3 constructs with the same total amount of DVL3 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. Individual p values were calculated with student t-tests and denote the difference between DVL-WT and the other conditions without WNT stimulation, difference between each condition with or without stimulation was also calculated. An asterisk indicates a significant difference: *: p < 0.05; **: p < 0.005; ***: p < 0.0005; ****: p < 0.0001.
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    (A): HEK293T cells were transiently transfected with firefly reporter plasmids for <t>SuperTopFlash</t> together with a fixed amount of each GFP tagged DVL1 construct or with a 1:1 stoichiometric ratio of two DVL1 constructs with the same total amount of DVL1 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (B): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each FLAG tagged DVL2 construct or with a 1:1 stoichiometric ratio of two DVL2 constructs with the same total amount of DVL2 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (C): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each HA tagged DVL3 construct or with a 1:1 stoichiometric ratio of two DVL3 constructs with the same total amount of DVL3 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. Individual p values were calculated with student t-tests and denote the difference between DVL-WT and the other conditions without WNT stimulation, difference between each condition with or without stimulation was also calculated. An asterisk indicates a significant difference: *: p < 0.05; **: p < 0.005; ***: p < 0.0005; ****: p < 0.0001.
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    (A): HEK293T cells were transiently transfected with firefly reporter plasmids for <t>SuperTopFlash</t> together with a fixed amount of each GFP tagged DVL1 construct or with a 1:1 stoichiometric ratio of two DVL1 constructs with the same total amount of DVL1 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (B): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each FLAG tagged DVL2 construct or with a 1:1 stoichiometric ratio of two DVL2 constructs with the same total amount of DVL2 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (C): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each HA tagged DVL3 construct or with a 1:1 stoichiometric ratio of two DVL3 constructs with the same total amount of DVL3 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. Individual p values were calculated with student t-tests and denote the difference between DVL-WT and the other conditions without WNT stimulation, difference between each condition with or without stimulation was also calculated. An asterisk indicates a significant difference: *: p < 0.05; **: p < 0.005; ***: p < 0.0005; ****: p < 0.0001.
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    (A): HEK293T cells were transiently transfected with firefly reporter plasmids for <t>SuperTopFlash</t> together with a fixed amount of each GFP tagged DVL1 construct or with a 1:1 stoichiometric ratio of two DVL1 constructs with the same total amount of DVL1 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (B): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each FLAG tagged DVL2 construct or with a 1:1 stoichiometric ratio of two DVL2 constructs with the same total amount of DVL2 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (C): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each HA tagged DVL3 construct or with a 1:1 stoichiometric ratio of two DVL3 constructs with the same total amount of DVL3 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. Individual p values were calculated with student t-tests and denote the difference between DVL-WT and the other conditions without WNT stimulation, difference between each condition with or without stimulation was also calculated. An asterisk indicates a significant difference: *: p < 0.05; **: p < 0.005; ***: p < 0.0005; ****: p < 0.0001.
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    (A): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each GFP tagged DVL1 construct or with a 1:1 stoichiometric ratio of two DVL1 constructs with the same total amount of DVL1 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (B): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each FLAG tagged DVL2 construct or with a 1:1 stoichiometric ratio of two DVL2 constructs with the same total amount of DVL2 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (C): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each HA tagged DVL3 construct or with a 1:1 stoichiometric ratio of two DVL3 constructs with the same total amount of DVL3 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. Individual p values were calculated with student t-tests and denote the difference between DVL-WT and the other conditions without WNT stimulation, difference between each condition with or without stimulation was also calculated. An asterisk indicates a significant difference: *: p < 0.05; **: p < 0.005; ***: p < 0.0005; ****: p < 0.0001.

    Journal: bioRxiv

    Article Title: Pathogenic DVL frameshifting variants in Robinow syndrome disrupt WNT signaling and cellular dynamics

    doi: 10.1101/2025.08.02.668297

    Figure Lengend Snippet: (A): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each GFP tagged DVL1 construct or with a 1:1 stoichiometric ratio of two DVL1 constructs with the same total amount of DVL1 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (B): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each FLAG tagged DVL2 construct or with a 1:1 stoichiometric ratio of two DVL2 constructs with the same total amount of DVL2 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (C): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each HA tagged DVL3 construct or with a 1:1 stoichiometric ratio of two DVL3 constructs with the same total amount of DVL3 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. Individual p values were calculated with student t-tests and denote the difference between DVL-WT and the other conditions without WNT stimulation, difference between each condition with or without stimulation was also calculated. An asterisk indicates a significant difference: *: p < 0.05; **: p < 0.005; ***: p < 0.0005; ****: p < 0.0001.

    Article Snippet: Cells were transfected using Lipofectamine 3000 (Invitrogen, Cat. No. L3000-008) with plasmids containing the wild type or variant DVL genes plus firefly reporter plasmids for SuperTopFlash (M50 Super 8x TOPFlash, Addgene plasmid #12456) and FOPFlash (M51 Super 8x FOPFlash, Addgene plasmid #12457) plasmid used as a negative control for “leaky” firefly luciferase transcription.

    Techniques: Transfection, Construct